Boster Immunoleader

antibody

Anti-B Raf Antibody

PA1848

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-B Raf Antibody

PA1848

Polyclonal

Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human B Raf(344-362aa QPFRPADEDHRNQFGQRDR), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

BRAF(v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

1. Eychene, A., Barnier, J. V., Apiou, F., Dutrillaux, B., Calothy, G. Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94(Braf/Rmil) and B-raf-2, a processed pseudogene.Oncogene 7: 1657-1660, 1992. 2. Hishinuma, A., Fukata, S., Kakudo, K., Murata, Y., Ieiri, T. High incidence of thyroid cancer in long-standing goiters with thyroglobulin mutations. Thyroid 15: 1079-1084, 2005. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.

BRAF

Serine/threonine-protein kinase B-raf

v-raf murine sarcoma viral oncogene homolog B1

FLJ95109 antibody 94 kDa B raf protein antibody B raf 1 antibody B Raf proto oncogene serine threonine protein kinase antibody BRAF 1 antibody BRAF antibody BRAF_HUMAN antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral(v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody

P15056