Boster Immunoleader

antibody

Anti-ABCA1 Antibody

PA1843

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-ABCA1 Antibody

PA1843

Polyclonal

Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family A member 1(ABCA1) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1(2219-2236aa NFAKDQSDDDHLKDLSLH), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

ABCA1(ATP-binding cassette, sub-family A(ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein(CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux  pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.

1. Langmann, T., Klucken, J., Reil, M., Liebisch, G., Luciani, M.-F., Chimini, G., Kaminski, W. E., Schmitz, G. Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages. Biochem. Biophys. Res. Commun. 257: 29-33, 1999. 2. Szakacs, G., Langmann, T., Ozvegy, C., Orso, E., Schmitz, G., Varadi, A., Sarkadi, B. Characterization of the ATPase cycle of human ABCA1: implications for its function as a regulator rather than an active transporter. Biochem. Biophys. Res. Commun. 288: 1258-1264, 2001.

ABCA1

ATP-binding cassette sub-family A member 1

ATP-binding cassette, sub-family A(ABC1), member 1

ABC 1 antibody ABC Transporter 1 antibody ABC-1 antibody ABC1 antibody ABCA 1 antibody ABCA1 antibody ABCA1_HUMAN antibody ATP binding Cassette 1 antibody ATP binding cassette sub family A ABC1 member 1 antibody ATP binding cassette sub family A member 1 antibody ATP binding cassette sub-family A member 1 antibody ATP binding Cassette Transporter 1 antibody ATP-binding Cassette 1 antibody ATP-binding cassette sub-family A member 1 antibody ATP-binding Cassette Transporter 1 antibody CERP antibody Cholesterol Efflux Regulatory Protein antibody FLJ14958 antibody HDLDT1 antibody Membrane bound antibody MGC164864 antibody MGC165011 antibody TD antibody TGD antibody

O95477