Boster Immunoleader

antibody

Anti-STAT5b Antibody

PA1841

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-STAT5b Antibody

PA1841

Polyclonal

Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 5B(STAT5B) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5b(767-787aa RRVEELLGRPMDSQWIPHAQS), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

STAT5B(Signal transducer and activator of transcription 5B) is a protein that in humans is encoded by the STAT5B gene. STAT5B orthologs have been identified in most placentals for which complete genome data are available. By FISH, Lin et al.(1996) mapped STAT5B to 17q11.2. The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha(RARA) gene in a small subset of acute promyelocytic leukemias(APML). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APML.

1. Ambrosio, R., Fimiani, G., Monfregola, J., Sanzari, E., De Felice, N., Salerno, M. C., Pignata, C., D'Urso, M., Ursini, M. V.The structure of human STAT5A and STAT5B genes reveals two regions of nearly identical sequence and an alternative tissue specific STAT5B promoter.Gene 285: 311-318, 2002. 2. Arnould, C., Philippe, C., Bourdon, V., Gregoire, M. J., Berger, R., Jonveaux, P.The signal transducer and activator of transcription STAT5b gene is a new partner of retinoic acid receptor alpha in acute promyelocytic-like leukaemia.Hum. Molec. Genet. 8: 1741-1749, 1999. 3. Lin, J.-X., Mietz, J., Modi, W. S., John, S., Leonard, W. J.Cloning of human Stat5B: reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells.J. Biol. Chem. 271: 10738-10744, 1996.

STAT5B

Signal transducer and activator of transcription 5B

signal transducer and activator of transcription 5B

Signal transducer and activator of transcription 5B antibody STA5B_HUMAN antibody STAT5 antibody Stat5b antibody Transcription factor STAT5B antibody

P51692