Boster Immunoleader

antibody

Anti-STAT5a Antibody

PA1840

100 ug/vial

200 USD

polyclonal

rabbit

Anti-STAT5a Antibody

PA1840

Polyclonal

Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 5A(STAT5A) detection. Tested with WB in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a(735-751aa YNMYPQNPDHVLDQDGE).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

STAT5(Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al.(1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al.(2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene.

1. Friedrichsen, B. N., Galsgaard, E. D., Nielsen, J. H., Moldrup, A. Growth hormone- and prolactin-induced proliferation of insulinoma cells, INS-1, depends on activation of STAT5 (signal transducer and activator of transcription 5). Molec. Endocr. 15: 136-148, 2001. 2. Hou, J., Schindler, U., Henzel, W. J., Wong, S. C., McKnight, S. L. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321-329, 1995. 3. Schwaller, J., Parganas, E., Wang, D., Cain, D., Aster, J. C., Williams, I. R., Lee, C.-K., Gerthner, R., Kitamura, T., Frantsve, J., Anastasiadou, E., Loh, M. L., Levy, D. E., Ihle, J. N., Gilliland, D. G. Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2.Molec. Cell 6: 693-704, 2000.

STAT5A

Signal transducer and activator of transcription 5A

signal transducer and activator of transcription 5A

Mammary gland factor antibody MGF antibody Signal transducer and activator of transcription 5A antibody STA5A_HUMAN antibody STAT 5 antibody STAT 5A antibody STAT5 antibody STAT5A antibody

P42229