Anti-SDHC Antibody | Boster Immunoleader PA1839 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SDHC Antibody

catalog :

PA1839

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-SDHC Antibody

Catalog Number :

PA1839

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Succinate dehydrogenase cytochrome b560 subunit, mitochondrial(SDHC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human SDHC(35-50aa KEEMERFWNKNIGSNR), different from the related mouse and rat sequences by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

SDHC(succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa) also known as SDH3, SUCCINATE DEHYDROGENASE CYTOCHROME b. The SDHC gene contains 6 exons. Oostveen et al.(1995) found that in fact it was protein from the bovine SDH3 gene(encoding 1 of the 2 integral membrane proteins) that complemented the hamster mutation. The authors localized the human SDH3 gene to the short arm of chromosome one, within 1 to 2 Mb from the centromere. Additionally, the authors stated that Southern analyses of human genomic DNA suggested that there are multiple SDH3 genes or pseudogenes. Elbehti-Green et al.(1998) confirmed the assignment of the SDHC gene to 1q21 by FISH. Baysal et al.(2004) described a family with PGL3 in which an 8,372-bp deletion in the SDHC gene was transmitted both maternally and paternally, without evidence of genomic imprinting.

Reference :

1. Baysal, B. E., Willett-Brozick, J. E., Filho, P. A. A., Lawrence, E. C., Myers, E. N., Ferrell, R. E. An Alu-mediated partial SDHC deletion causes familial and sporadic paraganglioma. J. Med. Genet. 41: 703-709, 2004. 2. Elbehti-Green, A., Au, H. C., Mascarello, J. T., Ream-Robinson, D., Scheffler, I. E. Characterization of the human SDHC gene encoding one of the integral membrane proteins of succinate-quinone oxidoreductase in mitochondria. Gene 213: 133-140, 1998. 3. Oostveen, F. G., Au, H. C., Meijer, P.-J., Scheffler, I. E. A Chinese hamster mutant cell line with a defect in the integral membrane protein C(II-3) of complex II of the mitochondrial electron transport chain. J. Biol. Chem. 270: 26104-26108, 1995.

Gene Name :

SDHC

Protein Name :

Succinate dehydrogenase cytochrome b560 subunit, mitochondrial

Gene Full Name :

succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa

Synonyms :

CYB560 antibody C560_HUMAN antibody CYBL antibody Cytochrome B large subunit of complex II antibody Integral membrane protein CII-3 antibody mitochondrial antibody PGL3 antibody QPs-1 antibody QPs1 antibody SDH3 antibody sdhC antibody Succinate dehydrogenase complex subunit C antibody Succinate dehydrogenase complex subunit C integral membrane protein 15kDa antibody Succinate dehydrogenase cytochrome b560 subunit antibody Succinate dehydrogenase cytochrome b560 subunit mitochondrial antibody Succinate dehydrogenase cytochrome b560 subunit, mitochondrial precursor antibody Succinate-ubiquinone oxidoreductase cytochrome B large subunit antibody

Uniprot ID :

Q99643

more info or order :

Boster Immunoleader product webpage