Anti-Peroxiredoxin 5 Antibody | Boster Immunoleader PA1838 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Peroxiredoxin 5 Antibody

catalog :

PA1838

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-Peroxiredoxin 5 Antibody

Catalog Number :

PA1838

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Peroxiredoxin-5, mitochondrial(PRDX5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 5(166-181aa DDSLVSIFGNRRLKRF), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

PRDX5(peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped on 11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Reference :

1. Budanov, A. V., Sablina, A. A., Feinstein, E., Koonin, E. V., Chumakov, P. M. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD. Science 304: 596-600, 2004. 2. Knoops, B., Clippe, A., Bogard, C., Arsalane, K., Wattiez, R., Hermans, C., Duconseille, E., Falmagne, P., Bernard, A. Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family. J. Biol. Chem. 274: 30451-30458, 1999. 3. Wang, M.-X., Wei, A., Yuan, J., Trickett, A., Knoops, B., Murrell, G. A. C. Expression and regulation of peroxiredoxin 5 in human osteoarthritis. FEBS Lett. 531: 359-362, 2002.

Gene Name :

PRDX5

Protein Name :

Peroxiredoxin-5, mitochondrial

Gene Full Name :

peroxiredoxin 5

Synonyms :

ACR 1 antibody ACR1 antibody Alu co repressor 1 antibody Alu co-repressor 1 antibody Alu corepressor 1 antibody antioxidant enzyme B166 antibody AOEB166 antibody B166 antibody liver tissue 2D-page spot 71B antibody MGC117264 antibody MGC142283 antibody MGC142285 antibody mitochondrial antibody Peroxiredoxin 5 mitochondrial antibody Peroxiredoxin 5, mitochondrial antibody Peroxiredoxin V antibody Peroxiredoxin-5 antibody Peroxiredoxin5 antibody peroxisomal antioxidant enzyme antibody PLP antibody PMP 20 antibody PMP20 antibody PRDX 5 antibody PRDX 6 antibody PRDX5 antibody PRDX5_HUMAN antibody PRDX6 antibody Prx V antibody Prx-V antibody PRXV antibody SBBI10 antibody thioredoxin peroxidase PMP20 antibody Thioredoxin reductase antibody TPx type VI antibody

Uniprot ID :

P30044

more info or order :

Boster Immunoleader product webpage