Anti-E2F6 Antibody | Boster Immunoleader PA1811 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-E2F6 Antibody

catalog :

PA1811

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-E2F6 Antibody

Catalog Number :

PA1811

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human E2F6(162-177aa KDCAQQLFELTDDKEN), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

E2F6(E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.

Reference :

1. Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D. M., Nakatani, Y. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science 296: 1132-1136, 2002. 2. Trimarchi, J. M., Fairchild, B., Verona, R., Moberg, K., Andon, N., Lees, J. A. E2F-6, a member of the E2F family that can behave as a transcriptional repressor. Proc. Nat. Acad. Sci. 95: 2850-2855, 1998.

Gene Name :

E2F6

Protein Name :

Transcription factor E2F6(E2F-6)

Gene Full Name :

E2F transcription factor 6

Synonyms :

E2F 6 antibody E2F binding site modulating activity protein antibody E2F transcription factor 6 antibody E2F transcription factor 6 isoform 1 antibody E2F-6 antibody E2F6 antibody E2F6_HUMAN antibody EMA antibody MGC111545 antibody Transcription factor E2F6 antibody

Uniprot ID :

O75461

more info or order :

Boster Immunoleader product webpage