Anti-CrkL Antibody | Boster Immunoleader PA1808 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CrkL Antibody

catalog :

PA1808

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CrkL Antibody

Catalog Number :

PA1808

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Crk-like protein(CRKL) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL(286-303aa FPFTHVKIFDPQNPDENE), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CRKL(v-crk sarcoma virus CT10 oncogene homolog(avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3(src homology) domains. By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al.(1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene. Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.

Reference :

1. Guris, D. L., Duester, G., Papaioannou, V. E., Imamoto, A. Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev. Cell 10: 81-92, 2006. 2. Moon, A. M., Guris, D. L., Seo, J., Li, L., Hammond, J., Talbot, A., Imamoto, A. Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev. Cell 10: 71-80, 2006. 3. Senechal, K., Halpern, J., Sawyers, C. L. The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J. Biol. Chem. 38: 23255-23261, 1996.

Gene Name :

CRKL

Protein Name :

Crk-like protein

Gene Full Name :

v-crk sarcoma virus CT10 oncogene homolog(avian)-like

Synonyms :

Crk L antibody Crk like protein antibody Crk-like protein antibody Crkl antibody CRKL_HUMAN antibody Crkol antibody HGNC:2363 antibody Oncogene CrkL antibody V crk avian sarcoma virus CT10 oncogene homolog like antibody v crk sarcoma virus CT10 oncogene homolog(avian) like antibody V crk sarcoma virus CT10 oncogene homolog avian like antibody

Uniprot ID :

P46109

more info or order :

Boster Immunoleader product webpage