Boster Immunoleader

antibody

Anti-Aurora B Antibody

PA1806

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

Anti-Aurora B Antibody

PA1806

Polyclonal

Rabbit IgG polyclonal antibody for Aurora kinase B(AURKB) detection. Tested with WB in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B(72-87aa QNKQPFTIDNFEIGRP), different from the related rat sequence by two anino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

AURKB(aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB.

1. Andrews, P. D., Ovechkina, Y., Morrice, N., Wagenbach, M., Duncan, K., Wordeman, L., Swedlow, J. R. Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004. 2. Katayama, H., Ota, T., Morita, K., Terada, Y., Suzuki, F., Katoh, O., Tatsuka, M. Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells. Gene 224: 1-7, 1998. 3. Ramadan, K., Bruderer, R., Spiga, F. M., Popp, O., Baur, T., Gotta, M., Meyer, H. H. Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin. Nature 450: 1258-1262, 2007.

AURKB

Aurora kinase B

aurora kinase B

AIK2 antibody AIM-1 antibody AIM1 antibody ARK-2 antibody ARK2 antibody AurB antibody AURKB antibody AURKB_HUMAN antibody Aurora 1 antibody Aurora and Ipl1 like midbody associated protein 1 antibody Aurora kinase B antibody Aurora related kinase 2 antibody Aurora- and Ipl1-like midbody-associated protein 1 antibody Aurora-B antibody Aurora-related kinase 2 antibody Aurora/IPL1 related kinase 2 antibody Aurora/IPL1-related kinase 2 antibody IPL1 antibody Serine/theronine kinase 12 antibody Serine/threonine protein kinase 12 antibody Serine/threonine-protein kinase 12 antibody Serine/threonine-protein kinase aurora-B antibody STK-1 antibody STK1 antibody STK12 antibody STK5 antibody

O70126