Anti-HSPB2 Antibody | Boster Immunoleader PA1802 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HSPB2 Antibody

catalog :

PA1802

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

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product information

Product Name :

Anti-HSPB2 Antibody

Catalog Number :

PA1802

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human HSPB2(102-118aa RHPQRLDRHGFVSREFC), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(F) and ICC.

Background :

Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein(HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

Reference :

1. Iwaki, A., Nagano, T., Nakagawa, M., Iwaki, T., Fukumaki, Y. Identification and characterization of the gene encoding a new member of the alpha-crystallin/Small hsp family, closely linked to the alpha-B-crystallin gene in a head-to-head manner. Genomics 45: 386-394, 1997.

Gene Name :

HSPB2

Protein Name :

Heat shock protein beta-2(HspB2)

Gene Full Name :

heat shock 27kDa protein 2(HspB2)

Synonyms :

DMPK-binding protein antibody Heat shock 27kD protein 2 antibody Heat shock 27kDa protein 2 antibody Heat shock protein beta-2 antibody Heat-shock protein beta-2 antibody Hs.78846 antibody HSP27 antibody HspB2 antibody HSPB2_HUMAN antibody LOH11CR1K antibody MGC133245 antibody MKBP antibody

Uniprot ID :

Q16082

more info or order :

Boster Immunoleader product webpage