Anti-HLA DMB Antibody | Boster Immunoleader PA1788 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HLA DMB Antibody

catalog :

PA1788

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Feng J, Li M, Gu W, Tang H, Yu S. The aberrant expression of HLA-DR in intrahepatic bile ducts in patients with biliary atresia: an immunohistochemistry and immune electron microscopy study. J Pediatr Surg. 2004;39:1658-62 pubmed

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product information

Product Name :

Anti-HLA DMB Antibody

Catalog Number :

PA1788

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for HLA class II histocompatibility antigen, DM beta chain(HLA-DMB) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB(81-100aa NQKDTLMQRLRNGLQNCATH).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

HLA-DMB(major histocompatibility complex, class II, DM beta), also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLA-DMB gene. The HLA-DMB gene is mapped on 6p21.32. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha(DMA) and a beta(DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP(class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.

Reference :

1. Fling, S. P., Arp, B., Pious, D. HLA-DMA and -DMB genes are both required for MHC class II/peptide complex formation in antigen-presenting cells. Nature 368: 554-558, 1994. 2. Vogt AB, Kropshofer H (1999). "HLA-DM - an endosomal and lysosomal chaperone for the immune system.". Trends Biochem. Sci.24 (4): 150–4.

Gene Name :

HLA-DMB

Protein Name :

HLA class II histocompatibility antigen, DM beta chain

Gene Full Name :

major histocompatibility complex, class II, DM beta

Synonyms :

class II antigen HLA-DM beta chain antibody class II histocompatibility antigen antibody Class II histocompatibility antigen M beta chain antibody D6S221E antibody DMB antibody HLA class II histocompatibility antigen DM beta chain antibody HLA-DM histocompatibility type, beta chain antibody M beta chain antibody Major histocompatibility complex class II DM beta antibody MHC class II antigen DMB antibody MHC class II antigen HLA DM beta chain antibody MHC class II HLA DMB antibody OTTHUMP00000029257 antibody Really interesting new gene 7 protein antibody RING 7 antibody RING7 antibody

Uniprot ID :

P28068

more info or order :

Boster Immunoleader product webpage