Anti-CISH Antibody | Boster Immunoleader PA1786 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CISH Antibody

catalog :

PA1786

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CISH Antibody

Catalog Number :

PA1786

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein(CISH) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CISH(241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

Reference :

1. Hu, G., Zhou, R., Liu, J., Gong, A.-Y., Eischeid, A. N., Dittman, J. W., Chen, X.-M. MicroRNA-98 and let-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in response to microbial challenge. J. Immun. 183: 1617-1624, 2009. 2. Khor, C. C., Vannberg, F. O., Chapman, S. J., Guo, H., Wong, S. H., Walley, A. J., Vukcevic, D., Rautanen, A., Mills, T. C., Chang, K.-C., Kam, K.-M., Crampin, A. C., and 23 others. CISH and susceptibility to infectious diseases. New Eng. J. Med. 362: 2092-2101, 2010. 3. Uchida, K., Yoshimura, A., Inazawa, J., Yanagisawa, K., Osada, H., Masuda, A., Saito, T., Takahashi, T., Miyajima, A., Takahashi, T. Molecular cloning of CISH, chromosome assignment to 3p21.3, and analysis of expression in fetal and adult tissues. Cytogenet. Cell Genet. 78: 209-212, 1997.

Gene Name :

CISH

Protein Name :

Cytokine-inducible SH2-containing protein(CIS)

Gene Full Name :

cytokine inducible SH2-containing protein

Synonyms :

BACTS2 antibody cis 1 antibody CIS antibody CIS-1 antibody CIS1 antibody CISH antibody CISH_HUMAN antibody Cytokine inducible inhibitor of signaling type 1B antibody Cytokine inducible SH2 containing protein antibody Cytokine-inducible SH2-containing protein antibody G18 antibody Protein G18 antibody SOCS antibody Suppressor of cytokine signaling antibody

Uniprot ID :

Q9NSE2

more info or order :

Boster Immunoleader product webpage