Anti-Aurora A Antibody | Boster Immunoleader PA1785 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Aurora A Antibody

catalog :

PA1785

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Aurora A Antibody

Catalog Number :

PA1785

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Aurora kinase A(AURKA) detection. Tested with WB in Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse Predicted to work with: rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse; Predicted Species: Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A(109-125aa QKTEDTKKRQWTLEDFD), different from the related rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.

Reference :

1. Bischoff, J. R., Plowman, G. D. The Aurora/lpl 1p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol. 9: 454-459, 1999. 2. Hutterer, A., Berdnik, D., Wirtz-Peitz, F., Zigman, M., Schleiffer, A., Knoblich, J. A. Mitotic activation of the kinase Aurora-A requires its binding partner Bora. Dev. Cell 11: 147-157, 2006. 3. Seki, A., Coppinger, J. A., Jang, C.-Y., Yates, J. R., III, Fang, G. Bora and the kinase Aurora A cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658, 2008.

Gene Name :

AURKA

Protein Name :

Aurora kinase A

Gene Full Name :

aurora kinase A

Synonyms :

AIK antibody ARK-1 antibody ARK1 antibody AURA antibody AURKA antibody Aurora 2 antibody Aurora A antibody Aurora Family Kinase 1 antibody aurora kinase A antibody Aurora Related Kinase 1 antibody Aurora-related kinase 1 antibody Aurora/IPL1 Like Kinase antibody Aurora/IPL1 Related Kinase 1 antibody Aurora/IPL1-related kinase 1 antibody AURORA2 antibody AYK1 antibody BRAK antibody Breast Tumor Amplified Kinase antibody Breast tumor-amplified kinase antibody BTAK antibody hARK1 antibody IAK antibody IPL1 Aurora Related Kinase 1 antibody IPL1 Related Kinase antibody MGC34538 antibody OTTHUMP00000031340 antibody OTTHUMP00000031341 antibody OTTHUMP00000031342 antibody OTTHUMP00000031343 antibody OTTHUMP00000031344 antibody OTTHUMP00000031345 antibody OTTHUMP00000166071 antibody OTTHUMP00000166072 antibody PPP1R47 antibody Protein phosphatase 1, regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine kinase antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody STK7 antibody

Uniprot ID :

P97477

more info or order :

Boster Immunoleader product webpage