Boster Immunoleader

antibody

Anti-Vinculin Antibody

PA1781

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Vinculin Antibody

PA1781

Polyclonal

Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al.(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater-than-95% sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, D. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell adhesion.Nature 430: 583-586, 2004. 2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992. 3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic cardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem. Biophys. Res. Commun. 349: 709-715, 2006.

VCL

Vinculin

vinculin

CMD1W antibody CMH15 antibody Metavinculin antibody MVCL antibody OTTHUMP00000019861 antibody OTTHUMP00000019862 antibody VCL antibody VINC antibody VINC_HUMAN antibody Vinculin antibody

P18206