Anti-VDAC/Porin Antibody | Boster Immunoleader PA1780 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-VDAC/Porin Antibody

catalog :

PA1780

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-VDAC/Porin Antibody

Catalog Number :

PA1780

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Voltage-dependent anion-selective channel protein 1(VDAC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse; Predicted Species: Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

The voltage-dependent anion channel(VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is though to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

Reference :

1. Blachly-Dyson, E., Zambronicz, E. B., Yu, W. H., Adams, V., McCabe, E. R. B., Adelman, J., Colombini, M., Forte, M.Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel.J. Biol. Chem. 268: 1835-1841, 1993. 2. Geisler, S., Holmstrom, K. M., Skujat, D., Fiesel, F. C., Rothfuss, O. C., Kahle, P. J., Springer, W.PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.Nature Cell Biol. 12: 119-131, 2010. 3. Huizing, M., Ruitenbeek, W., van den Heuvel, L. P., Dolce, V., Iacobazzi, V., Smeitink, J. A. M., Palmieri, F., Trijbels, J. M. F.Human mitochondrial transmembrane metabolite carriers: tissue distribution and its implication for mitochondrial disorders.J. Bioenerg. Biomembr. 30: 277-284, 1998.

Gene Name :

VDAC1

Protein Name :

Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1)

Gene Full Name :

voltage-dependent anion channel 1

Synonyms :

N2441 antibody OMP2 antibody POR1 antibody hVDAC1 antibody MGC111064 antibody Mitochondrial Porin antibody Outer mitochondrial membrane protein porin 1 antibody Plasmalemmal porin antibody Porin 31 HL antibody Porin 31HL antibody Porin 31HM antibody VDAC 1 antibody VDAC antibody VDAC-1 antibody VDAC1 antibody VDAC1_HUMAN antibody Voltage dependent anion channel 1 antibody Voltage dependent anion selective channel protein 1 antibody Voltage-dependent anion-selective channel protein 1 antibody YNL055C antibody YNL2441C antibody

Uniprot ID :

P21796

more info or order :

Boster Immunoleader product webpage