Anti-STAT3 Antibody | Boster Immunoleader PA1778 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-STAT3 Antibody

catalog :

PA1778

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-STAT3 Antibody

Catalog Number :

PA1778

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT3(751-770aa QFESLTFDMELTSECATSPM), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription factor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member of the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression of STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at its amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl terminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered glycolytic and oxidative phosphorylation activities characteristic of cancer cells.

Reference :

1. Akira, S., Nishio, Y., Inoue, M., Wang, X.-J., Wei, S., Matsusaka, T., Yoshida, K., Sudo, T., Naruto, M., Kishimoto, T. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway. Cell 77: 63-71, 1994. 2. Bhattacharya, S., Schindler, C. Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559, 2003. 3. Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I. O. ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Nat. Acad. Sci. 104: 17305-17310, 2007.

Gene Name :

STAT3

Protein Name :

Signal transducer and activator of transcription 3

Gene Full Name :

signal transducer and activator of transcription 3(acute-phase response factor)

Synonyms :

1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 antibody Signal Transductor and Activator of Transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody

Uniprot ID :

P40763

more info or order :

Boster Immunoleader product webpage