Boster Immunoleader

antibody

Anti-KMT2A/MLL Antibody

PA1770

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

Anti-KMT2A/MLL Antibody

PA1770

Polyclonal

Rabbit IgG polyclonal antibody for Histone-lysine N-methyltransferase MLL(MLL) detection. Tested with WB in Human;Mouse.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human KMT2A(1-15aa MAHSCRWRFPARPGT), identical to the related mouse sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

MLL(Myeloid/lymphoid or mixed Lineage Leukemia gene),also called MLL1, ALL1 or CXX7, is a frequent target for recurrent translocations in acute leukemias that may be characterized as acute myeloid leukemia(AML), acute lymphoblastic leukemia(ALL), or mixed lineage(biphenotypic) leukemia(MLL). The MLL gene encodes a DNA-binding protein that methylates histone H3 lys4(H3K4) and positively regulates expression of target genes, including multiple HOX genes. The MLL gene is mapped on 11q23.3. A leukemogenicMLL fusion protein that activates HOX expression had no effect on histone methylation, suggesting a distinct mechanism for gene regulation by MLLand MLL fusion proteins. The MLL gene encodes a large nuclear protein that is required for the maintenance of HOX gene expression. MLL is cleaved at 2 conserved sites to generate an N-terminal 320-kD fragment(N320) and a C-terminal 180-kD fragment(C180), which heterodimerize to stabilize the complex and confer its subnuclear destination. RNA interference-mediated knockdown of taspase-1 in HeLa cells resulted in the appearance of unprocessed MLL and the loss of proper HOX gene expression.

1. Armstrong, S. A., Staunton, J. E., Silverman, L. B., Pieters, R., den Boer, M. L., Minden, M. D., Sallan, S. E., Lander, E. S., Golub, T. R., Korsmeyer, S. J. MLL translocations specify a distinct gene expression profile that distinguishes a unique leukemia. Nature Genet. 30: 41-47, 2002. 2. Bruch, J., Wilda, M., Teigler-Schlegel, A., Harbott, J., Borkhardt, A., Metzler, M. Occurrence of an MLL/LAF4 fusion gene caused by the insertion ins(11;2)(q23;q11.2q11.2) in an infant with acute lymphoblastic leukemia. (Letter) Genes Chromosomes Cancer 37: 106-109, 2003. 3. Caligiuri, M. A., Strout, M. P., Oberkircher, A. R., Yu, F., de la Chapelle, A., Bloomfield, C. D. The partial tandem duplication of ALL1 in acute myeloid leukemia with normal cytogenetics or trisomy 11 is restricted to one chromosome. Proc. Nat. Acad. Sci. 94: 3899-3902, 1997.

MLL

Histone-lysine N-methyltransferase MLL

myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila)

Acute lymphocytic leukemia 1 antibody ALL 1 antibody ALL-1 antibody ALL1 antibody C-terminal cleavage product of 180 kDa antibody CXXC 7 antibody CXXC-type zinc finger protein 7 antibody CXXC7 antibody HRX antibody HTRX 1 antibody HTRX1 antibody KMT2A antibody Lysine N-methyltransferase 2A antibody MLL 1A antibody Mll antibody MLL cleavage product C180 antibody MLL1 antibody MLL1_HUMAN antibody MLL1A antibody Myeloid/lymphoid or mixed lineage leukemia antibody N-terminal cleavage product of 320 kDa antibody p180 antibody p320 antibody Trithorax homolog antibody Trithorax homolog Drosophila antibody Trithorax like protein antibody Trithorax-like protein antibody TRX 1 antibody TRX1 antibody Zinc finger protein HRX antibody

Q03164