Anti-Gli3 Antibody | Boster Immunoleader PA1766 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Gli3 Antibody

catalog :

PA1766

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

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product information

Product Name :

Anti-Gli3 Antibody

Catalog Number :

PA1766

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcriptional activator GLI3(GLI3) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3(40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product(relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus(ci) gene product(Ci), which regulates the Patched(pct), gooseberry(gsb), and decapentaplegic(dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

Reference :

1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early stages of limb development. (Letter) Nature 436: 277-281, 2005. 2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Molec. Genet. 11: 1129-1135, 2002. 3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

Gene Name :

GLI3

Protein Name :

Transcriptional activator GLI3

Gene Full Name :

GLI family zinc finger 3

Synonyms :

ACLS antibody DNA binding protein antibody GCPS antibody Gli 3 antibody GLI family zinc finger 3 antibody GLI Kruppel family member GLI 3 antibody GLI Kruppel family member GLI3(Greig cephalopolysyndactyly syndrome) antibody GLI Kruppel family member GLI3 antibody GLI3 antibody GLI3 C-terminally truncated form antibody GLI3 form of 190 kDa antibody GLI3 form of 83 kDa antibody GLI3 full length protein antibody GLI3-190 antibody GLI3-83 antibody GLI3_HUMAN antibody GLI3FL antibody Glioma associated oncogene family zinc finger 3 antibody Oncogene GLI3 antibody PAP A antibody PAPA 1 antibody PAPA antibody PAPA1 antibody PAPB antibody PHS antibody PPD IV antibody PPDIV antibody Transcriptional activator GLI3 antibody Transcriptional repressor GLI3R antibody Zinc finger protein GLI 3 antibody Zinc finger protein GLI3 antibody

Uniprot ID :

P10071

more info or order :

Boster Immunoleader product webpage