Anti-TRPC3 Antibody | Boster Immunoleader PA1753 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-TRPC3 Antibody

catalog :

PA1753

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-TRPC3 Antibody

Catalog Number :

PA1753

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Short transient receptor potential channel 3(TRPC3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPC3(836-851aa HSFNSILNQPTRYQQI), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.

Reference :

1. Becker, E. B. E., Oliver, P. L., Glitsch, M. D., Banks, G. T., Achilli, F., Hardy, A., Nolan, P. M., Fisher, E. M. C., Davies, K. E. A point mutation in TRPC3 causes abnormal Purkinje cell development and cerebellar ataxia in moonwalker mice. Proc. Nat. Acad. Sci. 106: 6706-6711, 2009. 2. Philipp, S., Strauss, B., Hirnet, D., Wissenbach, U., Mery, L., Flockerzi, V., Hoth, M. TRPC3 mediates T-cell receptor-dependent calcium entry in human T-lymphocytes. J. Biol. Chem. 278: 26629-26638, 2003. 3. van Rossum, D. B., Patterson, R. L., Sharma, S., Barrow, R. K., Kornberg, M., Gill, D. L., Snyder, S. H. Phospholipase C-gamma-1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature 434: 99-104, 2005.

Gene Name :

TRPC3

Protein Name :

Short transient receptor potential channel 3(TrpC3)

Gene Full Name :

transient receptor potential cation channel, subfamily C, member 3

Synonyms :

Htrp 3 antibody Htrp3 antibody MGC124333 antibody Mtrp3 antibody Receptor activated cation channel TRP3 antibody Short transient receptor potential channel 3 antibody Transient receptor potential cation channel subfamily C member 3 antibody Transient receptor potential channel 3 antibody TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody Transient receptor protein 3 antibody Trp related protein 3 antibody TRP3 antibody TrpC3 antibody Trrp3 antibody

Uniprot ID :

Q13507

more info or order :

Boster Immunoleader product webpage