Anti-INPPL1 Antibody | Boster Immunoleader PA1750 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-INPPL1 Antibody

catalog :

PA1750

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-INPPL1 Antibody

Catalog Number :

PA1750

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2(INPPL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human INPPL1(349-363aa QRDSQEDWTTFTHDR), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

INPPL1(Inositol polyphosphate phosphatase-like 1), also called SHIP2, encodes inositol polyphosphate-5 phosphatase-like 1, a protein that in addition to the phosphatase domain contains an SH2(src-homology domain 2) motif. INPPL1 is a member of the family of inositol triphosphate phosphatases. The INPPL1 gene is mapped on 11q13.4. Immunoblot analysis revealed that SHIP2 was widely expressed in fibroblast and nonhematopoietic tumor cell lines, unlike the SHIP protein, which was found only in cell lines of hematopoietic origin. Using human epithelial cell lines and primary human corneal and epidermal keratinocytes, Yu et al.(2008) showed that microRNA-184(MIR184) interfered with the ability of MIR205 to downregulate expression of SHIP2.SHIP2 may play a significant role in regulation of phosphatidylinositol 3-prime-kinase signaling by growth factors and insulin. By targeting the translation-initiating ATG codon and deleting the first 18 exons encoding Inppl1, Sleeman et al.(2005) generated Inppl1 -/- mice that were null for Inppl1 mRNA and protein.

Reference :

1. Habib, T., Hejna, J. A., Moses, R. E., Decker, S. J. Growth factors and insulin stimulate tyrosine phosphorylation of the 51C/SHIP2 protein. J. Biol. Chem. 273: 18605-18609, 1998. 2. Sleeman, M. W., Wortley, K. E., Lai, K.-M. V., Gowen, L. C., Kintner, J., Kline, W. O., Garcia, K., Stitt, T. N., Yancopoulos, G. D., Wiegand, S. J., Glass, D. J. Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity. Nature Med. 11: 199-205, 2005. 3. Yu, J., Ryan, D. G., Getsios, S., Oliveira-Fernandes, M., Fatima, A., Lavker, R. M. MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia. Proc. Nat. Acad. Sci. 105: 19300-19305, 2008.

Gene Name :

INPPL1

Protein Name :

Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2

Gene Full Name :

inositol polyphosphate phosphatase-like 1

Synonyms :

4 antibody 5-trisphosphate 5-phosphatase 2 antibody 51C protein antibody EC 3.1.3.n1 antibody inositol polyphosphate phosphatase like 1 antibody Inositol polyphosphate phosphatase like protein 1 antibody Inositol polyphosphate phosphatase-like protein 1 antibody INPPL-1 antibody INPPL1 antibody Phosphatidylinositol 3 antibody Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 2 antibody Protein 51C antibody SH2 domain containing inositol 5' phosphatase 2 antibody SH2 domain-containing inositol 5''-phosphatase 2 antibody SH2 domain-containing inositol phosphatase 2 antibody SHIP-2 antibody SHIP2 antibody SHIP2_HUMAN antibody

Uniprot ID :

O15357

more info or order :

Boster Immunoleader product webpage