product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-IL3 Antibody
catalog :
PA1748
quantity :
100 ug/vial
price :
200 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
product information
Product Name :
Anti-IL3 Antibody
Catalog Number :
PA1748
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, IHC-P in Human.
Price :
200 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human
Applications :
WB,IHC-P
Western blot :
Concentration: 0.1-0.5μg/ml; Tested Species: Human
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-1μg/ml; Tested Species: Human
Notes :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
A synthetic peptide corresponding to a sequence at the N-terminus of human IL3(119-140aa, KDGDWNEFRRKLTFYLKTLENA).
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Background :
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
Reference :
1. Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47 (1): 3–10. 2. Webb, G. C., Lee, J. S., Campbell, H. D., Young, I. G. The genes for interleukins 3 and 5 map to the same locus on mouse chromosome 11. Cytogenet. Cell Genet. 50: 107-110, 1989.
Gene Name :
IL3
Protein Name :
Interleukin-3(IL-3)
Gene Full Name :
interleukin 3(colony-stimulating factor, multiple)
Synonyms :
Colony stimulating factor multiple antibody Hematopoietic growth factor antibody IL 3 antibody IL-3 antibody IL3 antibody IL3_HUMAN antibody Interleukin 3(colony stimulating factor, multiple) antibody Interleukin 3 antibody Interleukin-3 antibody Mast cell growth factor antibody MCGF antibody MGC79398 antibody MGC79399 antibody Multi CSF antibody Multilineage colony stimulating factor antibody Multipotential colony stimulating factor antibody Multipotential colony-stimulating factor antibody OTTHUMP00000065963 antibody P cell stimulating factor antibody P-cell-stimulating factor antibody
Uniprot ID :
P08700
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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