Boster Immunoleader

antibody

Anti-ALK Antibody

PA1741

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-ALK Antibody

PA1741

Polyclonal

Rabbit IgG polyclonal antibody for ALK tyrosine kinase receptor(ALK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human ALK(1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.

1. Benharroch, D., Meguerian-Bedoyan, Z., Lamant, L., Amin, C., Brugieres, L., Terrier-Lacombe, M.-J., Haralambieva, E., Pulford, K., Pileri, S., Morris, S. W., Mason, D. Y., Delsol, G. ALK-positive lymphoma: a single disease with a broad spectrum of morphology. Blood 91: 2076-2084, 1998. 2. Chen, Y., Takita, J., Choi, Y. L., Kato, M., Ohira, M., Sanada, M., Wang, L., Soda, M., Kikuchi, A., Igarashi, T., Nakagawara, A., Hayashi, Y., Mano, H., Ogawa, S. Oncogenic mutations of ALK kinase in neuroblastoma. Nature 455: 971-974, 2008. 3. Englund, C., Loren, C. E., Grabbe, C., Varshney, G. K., Deleuil, F., Hallberg, B., Palmer, R. H. Jeb signals through the Alk receptor tyrosine kinase to drive visceral muscle fusion. Nature 425: 512-516, 2003.

ALK

ALK tyrosine kinase receptor

anaplastic lymphoma receptor tyrosine kinase

Alk antibody ALK tyrosine kinase receptor antibody ALK tyrosine kinase receptor precursor antibody ALK/EML4 fusion gene, included antibody ALK/NPM1 fusion gene, included antibody ALK_HUMAN antibody anaplastic lymphoma kinase(Ki-1) antibody Anaplastic lymphoma kinase antibody Anaplastic lymphoma kinase Ki 1 antibody Anaplastic lymphoma kinase Ki1 antibody Anaplastic lymphoma kinase p80 antibody anaplastic lymphoma receptor tyrosine kinase antibody CD 246 antibody CD246 antibody CD246 antigen antibody EC 2.7.10.1 antibody Ki 1 antibody Ki1 antibody mutant anaplastic lymphoma kinase antibody NBLST 3 antibody NBLST3 antibody Tcrz antibody TFG/ALK antibody

Q9UM73