Boster Immunoleader

antibody

Anti-CD146 Antibody

PA1736

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-CD146 Antibody

PA1736

Polyclonal

Rabbit IgG polyclonal antibody for Cell surface glycoprotein MUC18(MCAM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human CD146(584-600aa KKGKLPCRRSGKQEITL), different from the related rat and mouse sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CD146(cluster of differentiation 146) also known as the melanoma cell adhesion molecule(MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. By radiation hybrid analysis, Kuske and Johnson(1999) mapped the CD146 gene to chromosome 11q23.3. It is a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.

1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001. 2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999. 3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.

MCAM

Cell surface glycoprotein MUC18

melanoma cell adhesion molecule

A32 antigen antibody CD 146 antibody CD146 antibody CD146 antigen antibody Cell surface glycoprotein MUC18 antibody Cell surface glycoprotein P1H12 antibody Gicerin antibody MCAM antibody Melanoma adhesion molecule antibody Melanoma associated antigen A32 antibody Melanoma associated antigen MUC18 antibody Melanoma associated glycoprotein MUC18 antibody Melanoma cell adhesion molecule antibody Melanoma-associated antigen A32 antibody Melanoma-associated antigen MUC18 antibody MelCAM antibody MUC 18 antibody MUC18 antibody MUC18_HUMAN antibody S endo 1 antibody S endo 1 endothelial associated antigen antibody S-endo 1 endothelial-associated antigen antibody Sendo 1 endothelial associated antigen antibody Sendo1 antibody

P43121