Boster Immunoleader

antibody

Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 Antibody

PA1727

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 Antibody

PA1727

Polyclonal

Rabbit IgG polyclonal antibody for(3R)-hydroxyacyl-CoA dehydrogenase(HSD17B4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Hydroxysteroid(17-beta) Dehydrogenase 4(744-761aa NIMLSQKLQMILKDYAKL), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the D-bifunctional enzyme with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.

1. Green, V. L., Speirs, V., Landolt, A. M., Foy, P. M., Atkin, S. L.17-beta-hydroxysteroid dehydrogenase type 1, 2, 3, and 4 expression and enzyme activity in human anterior pituitary adenomas.J. Clin. Endocr. Metab. 84: 1340-1345, 1999. 2. Jiang, L. L., Miyazawa, S., Souri, M., Hashimoto, T.Structure of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein.J. Biochem. 121: 364-369, 1997. 3. Leenders, F., Dolez, V., Begue, A., Moller, G., Gloeckner, J., de Launoit, Y., Adamski, J.Structure of the gene for the human 17-beta-hydroxysteroid dehydrogenase type IV.Mammalian Genome 9: 1036-1041, 1998.

HSD17B4

Enoyl-CoA hydratase 2

hydroxysteroid(17-beta) dehydrogenase 4

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody 17 beta HSD 4 antibody 17 beta HSD IV antibody 17 beta hydroxysteroid dehydrogenase 4 antibody 17-beta-HSD 4 antibody 17-beta-hydroxysteroid dehydrogenase 4 antibody 17beta estradiol dehydrogenase type IV antibody 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody 3-alpha antibody 7-alpha antibody Beta hydroxyacyl dehydrogenase antibody Beta keto reductase antibody D 3 hydroxyacyl CoA dehydratase antibody D bifunctional protein antibody D bifunctional protein peroxisomal antibody D-bifunctional protein antibody DBP antibody DHB4_HUMAN antibody EDH17B4 antibody Enoyl-CoA hydratase 2 antibody Hsd17b4 antibody MFE 2 antibody MFE-2 antibody MPF-2 antibody Multifunctional protein 2 antibody Peroxisomal multifunctional enzyme type 2 antibody Peroxisomal multifunctional protein 2 antibody SDR8C1 antibody Short chain dehydrogenase/reductase family 8C member 1 antibody

F5HE57