Boster Immunoleader

antibody

Anti-Iba1 Antibody

PA1724

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Iba1 Antibody

PA1724

Polyclonal

Rabbit IgG polyclonal antibody for Allograft inflammatory factor 1(AIF1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Iba1(99-114aa ETFSYPDFLRMMLGKR), different from the related rat and mouse sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

AIF1(Allograft inflammatory factor 1), also known as IRT-1, is a protein that in humans is encoded by the AIF1 gene. This gene is induced by cytokines and interferon. Its protein product is though to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts. Using in vitro binding and coimmunoprecipitation assays, Autieri et al.(2003) determined that AIF1 interacts with actin. In unstimulated VSMCs, AIF1 colocalized with F-actin, but translocated to lamellipodia upon PDGF stimulation. VSMCs stably transduced with AIF1 retrovirus migrated 2.6-fold more rapidly in response to PDGF than control cells. AIF1 colocalized with RAC1, and AIF1-transduced VSMCs showed a constitutive and enhanced activation of RAC1. Autieri et al.(2003) concluded that AIF1 binds and polymerizes F-actin and regulates RAC1 activity and VSMC migration.

1. Autieri, M. V., Agrawal, N.IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein.J. Biol. Chem. 273: 14731-14737, 1998. 2. Autieri, M. V., Kelemen, S. E., Wendt, K. W. AIF-1 is an actin-polymerizing and Rac1-activating protein that promotes vascular smooth muscle cell migration.Circ. Res. 92: 1107-1114, 2003.

AIF1

Allograft inflammatory factor 1(AIF-1)

allograft inflammatory factor 1

AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody G1 antibody G1 putative splice variant of allograft inflamatory factor 1 antibody IBA 1 antibody IBA1 antibody interferon gamma responsive transcript antibody Interferon responsive transcript 1 antibody interferon responsive transcript factor 1 antibody Ionized calcium binding adapter molecule 1 antibody Ionized calcium-binding adapter molecule 1 antibody ionized calcium-binding adapter molecule antibody IRT 1 antibody IRT1 antibody Microglia response factor antibody MRF1 antibody Protein g1 antibody

P55008