Boster Immunoleader

antibody

Anti-Glucose Transporter GLUT4 Antibody

PA1722

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Glucose Transporter GLUT4 Antibody

PA1722

Polyclonal

Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Glucose Transporter GLUT4(491-509aa EQEVKPSTELEYLGPDEND), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

1. Birnbaum, M. J. : Identification of a novel gene encoding an insulin-responsive glucose transporter protein. Cell 57: 305-315, 1989. 2. Bell, G. I.; Kayano, T.; Buse, J. B.; Burant, C. F.; Takeda, J.; Lin, D.; Fukumoto, H.; Seino, S. : Molecular biology of mammalian glucose transporters. Diabetes Care 13: 198-208, 1990. 3. Garvey, W. T.; Maianu, L.; Zhu, J.-H.; Brechtel-Hook, G.; Wallace, P.; Baron, A. D. : Evidence for defects in the trafficking and translocation of GLUT4 glucose transporters in skeletal muscle as a cause of human insulin resistance. J. Clin. Invest. 101: 2377-2386, 1998.

SLC2A4

Solute carrier family 2, facilitated glucose transporter member 4

solute carrier family 2(facilitated glucose transporter), member 4

insulin-responsive antibody Glucose transporter GLUT 4 antibody Glucose transporter type 4 antibody Glucose transporter type 4 insulin responsive antibody GLUT 4 antibody GLUT-4 antibody GLUT4 antibody GTR4_HUMAN antibody kug antibody SLC 2A4 antibody SLC2A4 antibody solute carrier family 2(facilitated glucose transporter) member 4 antibody Solute carrier family 2, facilitated glucose transporter member 4 antibody

P14672