Boster Immunoleader

antibody

Anti-GABA Transporter 1/GAT 1 Antibody

PA1721

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-GABA Transporter 1/GAT 1 Antibody

PA1721

Polyclonal

Rabbit IgG polyclonal antibody for Sodium- and chloride-dependent GABA transporter 1(SLC6A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat Predicted to work with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human GABA Transporter 1(473-487aa WFYGVNRFYDNIQEM), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

SLC6A1(Solute Carrier family 6(Neurotransmitter transporter, Gaba), Member 1), also called GABATR or GABATHG, encodes a gamma-aminobutyric acid(GABA) transporter, which removes GABA from the synaptic cleft. The SLC6A1 gene contains 15 exons and is approximately 25 kb by Lam et al. And it is mapped on 3p25.3. Zomot et al.(2007) showed that introduction of a negatively charged amino acid at or near 1 of the 2 putative sodium binding sites of the GABA transporter GAT1 from rat brain(also calledSLC6A1) renders both net flux and exchange of GABA largely chloride independent. By in vitro functional expression studies, the SLC6A1 insertion promoter(2 copies of the sequence) had significantly greater promoter activity than the noninsertion(1 copy of the sequence) promoter, which in turn had greater activity than a promoterless control. Studies of postmortem hippocampus from 69 African American individuals showed that the SLC6A1promoter genotype predicted SLC6A1 RNA expression and that overall SLC6A1 expression decreased with age.

1. Cope, D. W., Di Giovanni, G., Fyson, S. J., Orban, G., Errington, A. C., Lorincz, M. L., Gould, T. M., Carter, D. A., Crunelli, V. Enhanced tonic GABA-A inhibition in typical absence epilepsy. Nature Med. 15: 1392-1398, 2009. 2. Huang, F., Shi, L. J., Heng, H. H. Q., Fei, J., Guo, L.-H. Assignment of the human GABA transporter gene (GABATHG) locus to chromosome 3p24-p25. Genomics 29: 302-304, 1995. 3. Zomot, E., Bendahan, A., Quick, M., Zhao, Y., Javitch, J. A., Kanner, B. I. Mechanism of chloride interaction with neurotransmitter:sodium symporters. Nature 449: 726-730, 2007.

SLC6A1

Sodium- and chloride-dependent GABA transporter 1(GAT-1)

solute carrier family 6(neurotransmitter transporter, GABA), member 1

GABATHG antibody GABATR antibody GABT 1 antibody GABT1 antibody GAT-1 antibody GAT1 antibody SC6A1_HUMAN antibody SLC6A1 antibody Sodium and chloride dependent GABA transporter 1 antibody Sodium- and chloride-dependent GABA transporter 1 antibody Solute carrier family 6(neurotransmitter transporter GABA) member 1 antibody Solute carrier family 6 member 1 antibody

P30531