Boster Immunoleader

antibody

Anti-eNOS Antibody

PA1712-1

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-eNOS Antibody

PA1712-1

Polyclonal

Rabbit IgG polyclonal antibody for Nitric oxide synthase, endothelial(NOS3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human eNOS(144-165 QAHEQRLQEVEAEVAATGTYQL), identical to the related mouse sequence, and different from the related rat sequence by one sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

NOS3(Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction andplatelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to coronary spasm. Fulton et al.(1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells(HUVECs) and human aortic vascular smooth muscle cells(HAOVSMCs) was inversely proportional to that of NOS3AS.

1. Ahsan, A., Norboo, T., Baig, M. A., Pasha, M. A. Q. Simultaneous selection of the wild-type genotypes of the G894T and 4B/4A polymorphisms of NOS3 associate with high-altitude adaptation. Ann. Hum. Genet. 69: 260-267, 2005. 2. Chen, C.-A., Wang, T.-Y., Varadharaj, S., Reyes, L. A., Hemann, C., Talukder, M. A. H., Chen, Y.-R., Druhan, L. J., Zweier, J. L. S-glutathionylation uncouples eNOS and regulates its cellular and vascular function. Nature 468: 1115-1118, 2010. 3. Fulton, D., Gratton, J.-P., McCabe, T. J., Fontana, J., Fujio, Y., Walsh, K., Franke, T. F., Papapetropoulos, A., Sessa, W. C. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 399: 597-601, 1999.

NOS3

Nitric oxide synthase, endothelial

nitric oxide synthase 3(endothelial cell)

cNOS antibody Constitutive NOS antibody EC NOS antibody EC-NOS antibody ecNOS antibody Endothelial nitric oxidase synthase antibody Endothelial nitric oxide synthase antibody Endothelial nitric oxide synthase 3 antibody Endothelial NOS antibody eNOS antibody Nitric oxide synthase 3(endothelial cell) antibody Nitric oxide synthase 3 antibody Nitric oxide synthase 3 endothelial cell antibody Nitric oxide synthase endothelial antibody Nitric oxide synthase, endothelial antibody NOS 3 antibody NOS III antibody NOS type III antibody NOS3 antibody NOS3_HUMAN antibody NOSIII antibody

P29474