Anti-MCM2 Antibody | Boster Immunoleader PA1711 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MCM2 Antibody

catalog :

PA1711

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MCM2 Antibody

Catalog Number :

PA1711

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Mouse; Predicted Species: Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2(884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Reference :

Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988. 2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res Commun 177 (1): 395–400.

Gene Name :

MCM2

Protein Name :

DNA replication licensing factor MCM2

Gene Full Name :

minichromosome maintenance complex component 2

Synonyms :

BM28 antibody CCNL 1 antibody CCNL1 antibody CDC like 1 antibody CDC like-1 antibody cdc19 antibody CDCL 1 antibody CDCL1 antibody Cell devision cycle like 1 antibody Cyclin like 1 antibody cyclin like-1 antibody D3S3194 antibody DNA replication licensing factor MCM2 antibody KIAA0030 antibody MCM 2 antibody MCM2 antibody MCM2 minichromosome maintenance deficient 2 mitotin antibody MCM2 minichromosome maintenance deficient 2 mitotin(S. cerevisiae) antibody MCM2 minichromosome maintenance deficient 2, mitotin antibody MCM2_HUMAN antibody MGC10606 antibody Minichromosome maintenance complex component 2 antibody Minichromosome maintenance deficient 2(mitotin) antibody Minichromosome maintenance deficient 2 mitotin antibody Minichromosome maintenance protein 2 antibody Minichromosome maintenance protein 2 homolog antibody Mitotin antibody Nuclear protein BM28 antibody OTTHUMP00000216047 antibody OTTHUMP00000216050 antibody

Uniprot ID :

P97310

more info or order :

Boster Immunoleader product webpage