Anti-SOCS3 Antibody | Boster Immunoleader PA1707 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SOCS3 Antibody

catalog :

PA1707

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Huang Y, Wang Y, Li X, Chen Z, Li X, Wang H, et al. Molecular mechanism of ER stress-induced gene expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in macrophages. FEBS J. 2015;282:2361-78 pubmed publisher

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product information

Product Name :

Anti-SOCS3 Antibody

Catalog Number :

PA1707

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human;Mouse.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Reference :

1. Babon, J. J., McManus, E. J., Yao, S., DeSouza, D. P., Mielke, L. A., Sprigg, N. S., Willson, T. A., Hilton, D. J., Nicola, N. A., Baca, M., Nicholson, S. E., Norton, R. S.The structure of SOCS3 reveals the basis of the extended SH2 domain function and identifies an unstructured insertion that regulates stability.Molec. Cell 22: 205-216, 2006. 2. Chen, Z., Laurence, A., Kanno, Y., Pacher-Zavisin, M., Zhu, B.-M., Tato, C., Yoshimura, A., Hennighausen, L., O'Shea, J. J.Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells.Proc. Nat. Acad. Sci. 103: 8137-8142, 2006. 3. Jo, D., Liu, D., Yao, S., Collins, R. D., Hawiger, J.Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis.Nature Med. 11: 892-898, 2005.

Gene Name :

SOCS3

Protein Name :

Suppressor of cytokine signaling 3(SOCS-3)

Gene Full Name :

suppressor of cytokine signaling 3

Synonyms :

ATOD4 antibody CIS 3 antibody CIS-3 antibody CIS3 antibody Cish3 antibody Cytokine induced SH2 protein 3 antibody Cytokine-inducible SH2 protein 3 antibody E2a Pbx1 target gene in fibroblasts 10 antibody EF 10 antibody MGC71791 antibody SOCS 3 antibody SOCS-3 antibody Socs3 antibody SOCS3_HUMAN antibody SSI 3 antibody SSI-3 antibody SSI3 antibody STAT induced STAT inhibitor 3 antibody STAT-induced STAT inhibitor 3 antibody Suppressor of cytokine signaling 3 antibody

Uniprot ID :

O14543

more info or order :

Boster Immunoleader product webpage