Boster Immunoleader

antibody

Anti-CD62E Antibody

PA1704

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-CD62E Antibody

PA1704

Polyclonal

Rabbit IgG polyclonal antibody for E-selectin(SELE) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human CD62E(33-50aa YDEASAYCQQRYTHLVAI), different from the related rat and mouse sequences by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

E-Selectin(SELE), also called Selectin E, ELAM1 or ELAM, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. It is though to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. E-Selection is a member of the selectin family of cell adhesion molecules. The E-Selectin gene is mapped on 1q24.2. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA by Collins et al. Using affinity chromatography, pull-down assays, and mass spectrometric analysis on adenocarcinoma cell lines, Gout et al. showed that SELE bound death receptor-3 on the cancer cells. Western blot analysis revealed that the SELE-binding protein was recognized by anti-DR3 antibodies. Anti-DR3 or knockdown of DR3 by small interfering RNA decreased adhesion of colon cancer cells to SELE and to SELE-expressing endothelial cells.

1. Bevilacqua, M. P., Stengelin, S., Gimbrone, M. A., Jr., Seed, B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243: 1160-1165, 1989. 2. Chang, Y.-P. C., Liu, X., Kim, J. D. O., Ikeda, M. A., Layton, M. R., Weder, A. B., Cooper, R. S., Kardia, S. L. R., Rao, D. C., Hunt, S. C., Luke, A., Boerwinkle, E., Chakravarti, A. Multiple genes for essential-hypertension susceptibility on chromosome 1q. Am. J. Hum. Genet. 80: 253-264, 2007. 3. DeLisser, H. M., Christofidou-Solomidou, M., Sun, J., Nakada, M. T., Sullivan, K. E. Loss of endothelial surface expression of E-selectin in a patient with recurrent infections. Blood 94: 884-894, 1999.

SELE

E-selectin

selectin E

CD 62E antibody CD26E antibody CD62 antigen-like family member E antibody CD62E antibody CD62E antigen antibody E selectin antibody E-selectin antibody ELAM 1 antibody ELAM antibody ELAM-1 antibody ELAM1 antibody Endothelial adhesion molecule 1 antibody Endothelial leukocyte adhesion molecule 1 antibody ESEL antibody LECAM 2 antibody LECAM2 antibody Leukocyte endothelial cell adhesion molecule 2 antibody Leukocyte-endothelial cell adhesion molecule 2 antibody LYAM2_HUMAN antibody SELE antibody Selectin E antibody SelectinE antibody

P16581