Anti-Histidine decarboxylase Antibody | Boster Immunoleader PA1703 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Histidine decarboxylase Antibody

catalog :

PA1703

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Zhang A, Chen D, Wei H, Du L, Zhao T, Wang X, et al. Functional characterization of TNF-α in grass carp head kidney leukocytes: induction and involvement in the regulation of NF-κB signaling. Fish Shellfish Immunol. 2012;33:1123-32 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Histidine decarboxylase Antibody

Catalog Number :

PA1703

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Histidine decarboxylase(HDC) detection. Tested with WB, IHC-P in Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Histidine decarboxylase(620-639aa EQMMMMKKGAFKKLIKFYSV), different from the related rat sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

HDC(Histidine decarboxylase) is the enzyme that catalyzes the reaction that produces histamine from histidine with the help of vitamin B6. The HDC gene is mapped on 15q21.2. The deduced 662-amino acid protein has a molecular mass of 74,148 Da. The gene contains 12 exons spanning approximately 24 kb. The biogenic amine histamine is an important modulator of numerous physiologic processes, including neurotransmission, gastric acid secretion, and smooth muscle tone. The biosynthesis of histamine from histidine is catalyzed by the enzyme L-histidine decarboxylase. This homodimeric enzyme is a pyridoxal phosphate(PLP)-dependent decarboxylase and is highly specific for its histidine substrate. Ercan-Sencicek et al. noted that animal studies had shown that lack of Hdc in mice results in increased locomotor and stereotypic behaviors, as well as increased anxiety.

Reference :

1. Bruneau, G., Nguyen, V. C., Gros, F., Bernheim, A., Thibault, J. Preparation of a rat brain histidine decarboxylase (HDC) cDNA probe by PCR and assignment of the human HDC gene to chromosome 15. Hum. Genet. 90: 235-238, 1992. 2. Dere, E., De Souza-Silva, M. A., Spieler, R. E., Lin, J. S., Ohtsu, H., Haas, H. L., Huston, J. P. Changes in motoric, exploratory and emotional behaviours and neuronal acetylcholine content and 5-HT turnover in histidine decarboxylase-KO mice.Europ. J. Neurosci. 20: 1051-1058, 2004. 3. Ercan-Sencicek, A. G., Stillman, A. A., Ghosh, A. K., Bilguvar, K., O'Roak, B. J., Mason, C. E., Abbott, T., Gupta, A., King, R. A., Pauls, D. L., Tischfield, J. A., Heiman, G. A., and 16 others. L-histidine decarboxylase and Tourette's syndrome. New Eng. J. Med. 362: 1901-1908, 2010.

Gene Name :

HDC

Protein Name :

Histidine decarboxylase(HDC)

Gene Full Name :

histidine decarboxylase

Synonyms :

DCHS_HUMAN antibody EC 4.1.1.22 antibody HDC antibody Histidine decarboxylase antibody

Uniprot ID :

P23738

more info or order :

Boster Immunoleader product webpage