Boster Immunoleader

antibody

Anti-XRCC3 Antibody

PA1697

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

western blot, immunohistochemistry - paraffin section

info changed 20150508

Anti-XRCC3 Antibody

PA1697

Polyclonal

Rabbit IgG polyclonal antibody for DNA repair protein XRCC3(XRCC3) detection. Tested with WB, IHC-P in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse; Predicted Species: Rat

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of mouse XRCC3(290-305aa NQLLMRLMVDRTHEDD), identical to the related rat sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

XRCC3(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 3) is a DNA repair protein that in humans, is encoded by the XRCC3 gene. The XRCC3 is a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 interacts directly with RAD51 and may cooperate with RAD51 during recombinational repair. XRCC3 function is not limited to HR initiation, but extends to later stages in formation and resolution of HR intermediates, possibly by stabilizing heteroduplex DNA. The gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

1. Brenneman, M. A., Wagener, B. M., Miller, C. A., Allen, C., Nickoloff, J. A. XRCC3 controls the fidelity of homologous recombination: roles for XRCC3 in late stages of recombination. Molec. Cell 10: 387-395, 2002. 2. Kuschel, B., Auranen, A., McBride, S., Novik, K. L., Antoniou, A., Lipscombe, J. M., Day, N. E., Easton, D. F., Ponder, B. A. J., Pharoah, P. D. P., Dunning, A. Variants in DNA double-strand break repair genes and breast cancer susceptibility. Hum. Molec. Genet. 11: 1399-1407, 2002. 3. Tebbs RS, Zhao Y, Tucker JD, Scheerer JB, Siciliano MJ, Hwang M, Liu N, Legerski RJ, Thompson LH (Aug 1995). "Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene".

XRCC3

DNA repair protein XRCC3

X-ray repair complementing defective repair in Chinese hamster cells 3

CMM6 antibody DNA repair protein XRCC3 antibody RAD51 like antibody X ray repair complementing defective repair in Chinese hamster antibody X ray repair complementing defective repair in Chinese hamster cells 3 antibody X ray repair cross complementing protein 3 antibody X-ray repair cross-complementing protein 3 antibody XRCC 3 antibody XRCC3 antibody XRCC3_HUMAN antibody

Q9CXE6