Boster Immunoleader

antibody

Anti-PI 3 Kinase p85 beta Antibody

PA1680

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-PI 3 Kinase p85 beta Antibody

PA1680

Polyclonal

Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human PI 3 Kinase p85 beta(447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

PIK3R2(Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. The NS1 protein from various influenza strains also bound p85-beta, but not p85-alpha. Binding of p85-beta and activation of PI3K required tyr89 of NS1, and mutant viruses expressing NS1 with a tyr89-to-phe substitution grew more slowly in cell culture than wildtype viruses. Using mouse embryonic fibroblasts, Park et al. showed that, in addition to regulating PI3K function, p85-alpha and p85-beta regulated the function of Xbp1s, a transcription factor that orchestrates the unfolded protein response(UPR) following endoplasmic reticulum(ER) stress. Overexpression of PIK3R2 in livers of ob/ob mice increased glucose tolerance and reduced blood glucose concentrations.

1. Hale, B. G., Jackson, D., Chen, Y.-H., Lamb, R. A., Randall, R. E. Influenza A virus NS1 protein binds p85-beta and activates phosphatidylinositol-3-kinase signaling. Proc. Nat. Acad. Sci. 103: 14194-14199, 2006. 2. Oak, J. S., Deane, J. A., Kharas, M. G., Luo, J., Lane, T. E., Cantley, L. C., Fruman, D. A. Sjogren's syndrome-like disease in mice with T cells lacking class 1A phosphoinositide-3-kinase. Proc. Nat. Acad. Sci. 103: 16882-16887, 2006.

PIK3R2

Phosphatidylinositol 3-kinase regulatory subunit beta(PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta)

phosphoinositide-3-kinase, regulatory subunit 2(beta)

p85 antibody p85 beta antibody P85B antibody P85B_HUMAN antibody Phosphatidylinositol 3 kinase antibody Phosphatidylinositol 3 kinase regulatory beta subunit antibody Phosphatidylinositol 3 kinase regulatory subunit beta antibody Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2(p85 beta) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody Phosphatidylinositol 3-kinase regulatory subunit beta antibody Phosphoinositide 3 kinase regulatory subunit 2(beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2(p85 beta) antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody Phosphoinositide 3 kinase, regulatory subunit 2(beta) antibody Phosphoinositide 3 kinase, regulatory subunit 2(p85 beta) antibody PI3 kinase p85 beta subunit antibody PI3 kinase p85 subunit beta antibody PI3-kinase regulatory subunit beta antibody PI3-kinase subunit p85-beta antibody PI3K antibody PI3K regulatory subunit beta antibody PIK3R 2 antibody PIK3R2 antibody PtdIns 3 kinase p85 beta antibody PtdIns-3-kinase regulatory subunit beta antibody PtdIns-3-kinase regulatory subunit p85-beta antibody

O00459