Anti-ERp57 Antibody | Boster Immunoleader PA1679 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ERp57 Antibody

catalog :

PA1679

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ERp57 Antibody

Catalog Number :

PA1679

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Protein disulfide-isomerase A3(PDIA3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human ERp57(172-188aa LKAASNLRDNYRFAHTN), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

PDIA3(Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme, mapped on 15q15.3. The PDIA3 protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Koivunen et al. showed that PDIA3 could not substitute for the beta subunit of PDI. When coexpressed with alpha prolyl 4-hydroxylase, PDIA3 did not form prolyl 4-hydroxylase tetramers, nor did it have prolyl 4-hydroxylase activity. Hirano et al. expressed human PDIA3 and found that the protein had a thiol-dependent reductase activity. They showed that the expression level of PDIA3 is increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells.

Reference :

1. Bourdi, M., Demady, D., Martin, J. L., Jabbour, S. K., Martin, B. M., George, J. W., Pohl, L. R. cDNA cloning and baculovirus expression of the human liver endoplasmic reticulum P58: characterization as a protein disulfide isomerase isoform, but not as a protease or a carnitine acyltransferase. Arch. Biochem. Biophys. 323: 397-403, 1995. 2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006 3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006.

Gene Name :

PDIA3

Protein Name :

Protein disulfide-isomerase A3

Gene Full Name :

protein disulfide isomerase family A, member 3

Synonyms :

58 kDa glucose regulated protein antibody 58 kDa glucose-regulated protein antibody 58 kDa microsomal protein antibody Disulfide isomerase ER 60 antibody Disulfide isomerase ER-60 antibody Endoplasmic reticulum resident protein 57 antibody Endoplasmic reticulum resident protein 60 antibody ER p57 antibody ER protein 57 antibody ER protein 60 antibody ERp 57 antibody ERp57 antibody ERp60 antibody ERp61 antibody Glucose Regulated Protein 58 Kd antibody GRP 57 antibody GRP 58 antibody GRP57 antibody GRP58 antibody HsT17083 antibody P58 antibody PDIA 3 antibody PDIA3 antibody PDIA3_HUMAN antibody Phospholipase C alpha antibody PI PLC antibody Protein disulfide isomerase A3 antibody Protein disulfide isomerase family A member 3 antibody Protein disulfide-isomerase A3 antibody

Uniprot ID :

P30101

more info or order :

Boster Immunoleader product webpage