Anti-PDGF Receptor alpha Antibody | Boster Immunoleader PA1678 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PDGF Receptor alpha Antibody

catalog :

PA1678

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-PDGF Receptor alpha Antibody

Catalog Number :

PA1678

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Platelet-derived growth factor receptor alpha(PDGFRA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human PDGF Receptor alpha(1030-1043aa EEEDLGKRNRHSSQ), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

PDGFRA(Platelet-derived growth factor receptor, alpha), also called PDGFR2, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The PDGFA gene is mapped on 4q12. The PDGFRA-FIP1L1 gene is a constitutively activated tyrosine kinase that transforms hematopoietic cells and is a therapeutic target of imatinib. The PDGFRA gene contains 23 exons spanning about 65 kb. Using the human PDGFRA promoter linked to a luciferase reporter, Joosten et al. showed that PAX1 acts as a transcriptional activator of the PDGFRA gene in differentiated human embryonal carcinoma cells. PDGFRA is responsible for mediating cellular contraction of multiple growth factors: TGFB1 and members of the PDGF family. Lei et al. noted that in the rabbit model of the disease, PDGFRA is dramatically more capable of promoting PVR than is the closely related PDGFRB. PDGFRA is a critical receptor required for human CMV infection, and thus a target for novel antiviral therapies.

Reference :

1. Baxter, E. J., Hochhaus, A., Bolufer, P., Reiter, A., Fernandez, J. M., Senent, L., Cervera, J., Moscardo, F., Sanz, M. A., Cross, N. C. P. The t(4;22)(q12;q11) in atypical chronic myeloid leukaemia fuses BCR to PDGFRA. Hum. Molec. Genet. 11: 1391-1397, 2002. 2. Chen, H., Gu, X., Liu, Y., Wang, J., Wirt, S. E., Bottino, R., Schorle, H., Sage, J., Kim, S, K. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478: 349-355, 2011. 3. Chompret, A., Kannengiesser, C., Barrois, M., Terrier, P., Dahan, P., Tursz, T., Lenoir, G. M., Bressac-De Paillerets, B. PDGFRA germline mutation in a family with multiple cases of gastrointestinal stromal tumor. Gastroenterology 126: 318-321, 2004.

Gene Name :

PDGFRA

Protein Name :

Platelet-derived growth factor receptor alpha(PDGF-R-alpha/PDGFR-alpha)

Gene Full Name :

platelet-derived growth factor receptor, alpha polypeptide

Synonyms :

Alpha platelet derived growth factor receptor antibody Alpha-type platelet-derived growth factor receptor antibody CD 140a antibody CD140 antigen-like family member A antibody CD140a antibody CD140a antigen antibody MGC74795 antibody PDGF alpha chain antibody PDGF R alpha antibody PDGF-R-alpha antibody PDGFR 2 antibody PDGFR A antibody PDGFR alpha antibody PDGFR2 antibody PDGFRA antibody PDGFRA/BCR fusion antibody PGFRA_HUMAN antibody Platelet derived growth factor receptor 2 antibody Platelet derived growth factor receptor alpha antibody Platelet derived growth factor receptor alpha polypeptide antibody Platelet derived growth factor receptor antibody Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody RHEPDGFRA antibody

Uniprot ID :

P16234

more info or order :

Boster Immunoleader product webpage