Anti-CIAS1/NALP3 Antibody | Boster Immunoleader PA1665 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CIAS1/NALP3 Antibody

catalog :

PA1665

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Wang B, Yang G, Liang X, Zhu M, Du M. Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice. BMC Complement Altern Med. 2014;14:162 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CIAS1/NALP3 Antibody

Catalog Number :

PA1665

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3(NLRP3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3(NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Reference :

1. Agostini, L., Martinon, F., Burns, K., McDermott, M. F., Hawkins, P. N., Tschopp, J. NALP3 forms an IL-1-beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. 2. Allen, I. C., Scull, M. A., Moore, C. B., Holl, E. K., McElvania-TeKippe, E., Taxman, D. J., Guthrie, E. H., Pickles, R. J., Ting, J. P.-Y. The NLRP3 inflammasome mediates in vivo innate immunity to influenza A virus through recognition of viral RNA. Immunity 30: 556-565, 2009. 3. Hoffman HM, Wright FA, Broide DH, Wanderer AA, Kolodner RD (Jun 2000). "Identification of a locus on chromosome 1q44 for familial cold urticaria". Am J Hum Genet 66 (5): 1693–8.

Gene Name :

NLRP3

Protein Name :

NACHT, LRR and PYD domains-containing protein 3

Gene Full Name :

NLR family, pyrin domain containing 3

Synonyms :

AGTAVPRL antibody AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like antibody Angiotensin/vasopressin receptor AII/AVP-like antibody C1orf7 antibody Caterpiller protein 1.1 antibody CIAS 1 antibody CIAS1 antibody CLR1.1 antibody Cold autoinflammatory syndrome 1 antibody Cold autoinflammatory syndrome 1 protein antibody Cryopyrin antibody Familial cold autoinflammatory syndrome antibody FCAS antibody FCU antibody LRR and PYD domains-containing protein 3 antibody Muckle-Wells syndrome antibody MWS antibody NACHT antibody NACHT LRR and PYD containing protein 3 antibody NALP 3 antibody NALP3 antibody NALP3_HUMAN antibody NLRP3 antibody PYPAF 1 antibody PYPAF1 antibody PYRIN containing APAF1 like protein 1 antibody PYRIN-containing APAF1-like protein 1 antibody

Uniprot ID :

Q96P20

more info or order :

Boster Immunoleader product webpage