Boster Immunoleader

antibody

Anti-NFAT1 Antibody

PA1664

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-NFAT1 Antibody

PA1664

Polyclonal

Rabbit IgG polyclonal antibody for Nuclear factor of activated T-cells, cytoplasmic 2(NFATC2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human NFAT1(895-913aa KQEQNLDQTYLDDVNEIIR), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

NFATC2(Nuclear factor of activated T-cells, cytoplasmic 2),also known as NFATP or the "preexisting component" of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T cells(NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFATC2 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of NFATC2 promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive(TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL.

1. Aramburu, J., Garcia-Cozar, F., Raghavan, A., Okamura, H., Rao, A., Hogan, P. G. Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT. Molec. Cell 1: 627-637, 1998. 2. Baksh, S., Widlund, H. R., Frazer-Abel, A. A., Du, J., Fosmire, S., Fisher, D. E., DeCaprio, J. A., Modiano, J. F., Burakoff, S. J. NFATc2-mediated repression of cyclin-dependent kinase 4 expression. Molec. Cell 10: 1071-1081, 2002. 3. Northrop JP, Ho SN, Chen L, Thomas DJ, Timmerman LA, Nolan GP, Admon A, Crabtree GR (Jul 1994). "NF-AT components define a family of transcription factors targeted in T-cell activation". Nature 369 (6480): 497–502.

NFATC2

Nuclear factor of activated T-cells, cytoplasmic 2(NF-ATc2/NFATc2)

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

AI607462 antibody cytoplasmic 2 antibody KIAA0611 antibody NF ATp antibody NF-ATc2 antibody NF-ATp antibody NFAC2_HUMAN antibody NFAT 1 antibody NFAT pre existing subunit antibody NFAT pre-existing subunit antibody NFAT transcription complex, preexisting component antibody NFAT1 antibody NFAT1-D antibody NFATc2 antibody NFATp antibody Nuclear factor of activated T cells cytoplasmic 2 antibody Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody Nuclear factor of activated T cells pre-existing component antibody Nuclear factor of activated T-cells antibody T cell transcription factor NFAT 1 antibody T-cell transcription factor NFAT1 antibody

Q13469