Boster Immunoleader

antibody

Anti-Netrin 1 Antibody

PA1662

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Netrin 1 Antibody

PA1662

Polyclonal

Rabbit IgG polyclonal antibody for Netrin-1(NTN1) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1(32-51aa AGQAAQPDPCSDENGHPRRC), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Netrin-1(NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

1. Colon-Ramos, D. A., Margeta, M. A., Shen, K. Glia promote local synaptogenesis through UNC-6 (netrin) signaling in C. elegans. Science 318: 103-106, 2007. 2. Corset, V., Nguyen-Ba-Charvet, K. T., Forcet, C., Moyse, E., Chedotal, A., Mehlen, P. Netrin-1-mediated axon outgrowth and cAMP production requires interaction with adenosine A2b receptor. Nature 407: 747-750, 2000. 3. Powell AW, Sassa T, Wu Y, Tessier-Lavigne M, Polleux F (May 2008). Ghosh, Anirvan. ed. "Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon.

NTN1

Netrin-1

netrin 1

NET1_HUMAN antibody Netrin 1, mouse, homolog of antibody Netrin 1-like antibody Netrin-1 antibody Netrin1 antibody Ntn 1 antibody NTN 1L antibody Ntn1 antibody NTN1L antibody Unc6 antibody

O95631