Boster Immunoleader

antibody

Anti-Chk2 Antibody

PA1655

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Chk2 Antibody

PA1655

Polyclonal

Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Chk2(CHEK2) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Chk2(133-148aa TDKYRTYSKKHFRIFR), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

CHEK2(checkpoint kinase 2), also called CHK2 or RAD53, is a cell cycle checkpoint regulator and putative tumor suppressor which contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. CHEK2 is mapped on 22q12.1. CHEK2 was modified by phosphorylation and activated in response to ionizing radiation, and was also modified in response to hydroxyurea treatment. In response to DNA damage, CHEK2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Following gamma irradiation, CHEK2 phosphorylated PML on ser117, causing dissociation of the 2 proteins.

1. Ahn, J.-Y., Li, X., Davis, H. L., Canman, C. E. Phosphorylation of threonine 68 promotes oligomerization and autophosphorylation of the Chk2 protein kinase via the forkhead-associated domain. J. Biol. Chem. 277: 19389-19395, 2002. 2. Bartkova, J., Horejsi, Z., Koed, K., Kramer, A., Tort, F., Zieger, K., Guldberg, P., Sehested, M., Nesland, J. M., Lukas, C., Orntoft, T., Lukas, J., Bartek, J. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434: 864-870, 2005. 3. Bell, D. W., Varley, J. M., Szydlo, T. E., Kang, D. H., Wahrer, D. C. R., Shannon, K. E., Lubratovich, M., Verselis, S. J., Isselbacher, K. J., Fraumeni, J. F., Birch, J. M., Li, F. P., Garber, J. E., Haber, D. A. Heterozygous germ line hCHK2 mutations in Li-Fraumeni syndrome. Science 286: 2528-2531, 1999.

CHEK2

Serine/threonine-protein kinase Chk2

checkpoint kinase 2

bA444G7 antibody CDS 1 antibody CDS1 antibody Checkpoint kinase 2 antibody Checkpoint like protein CHK2 antibody Chek 2 antibody Chek2 antibody Chk 2 antibody CHK2 checkpoint homolog(S. pombe) antibody CHK2 checkpoint homolog antibody CHK2_HUMAN antibody HuCds 1 antibody HuCds1 antibody LFS 2 antibody LFS2 antibody PP1425 antibody RAD 53 antibody RAD53 antibody Rad53 homolog antibody Serine/threonine protein kinase Chk2 antibody Serine/threonine-protein kinase Chk2 antibody

O96017