Anti-MCM4 Antibody | Boster Immunoleader PA1652 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MCM4 Antibody

catalog :

PA1652

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MCM4 Antibody

Catalog Number :

PA1652

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM4(MCM4) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human MCM4(815-829aa TPALKYQQLFEDIRG), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

MCM4(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 4), also called CDC21, S. POMBE, HOMOLOG OF, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM4 gene is mapped to 8q11.21. The 864-amino acid MCM4 protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex(also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

Reference :

1. Connelly, M. A., Zhang, H., Kieleczawa, J., Anderson, C. W. The promoters for human DNA-PK(cs) (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11. Genomics 47: 71-83, 1998. 2. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 3. Hu B, Burkhart R, Schulte D et al (1994). "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins". Nucleic Acids Res. 21 (23): 5289–93.

Gene Name :

MCM4

Protein Name :

DNA replication licensing factor MCM4

Gene Full Name :

minichromosome maintenance complex component 4

Synonyms :

19G antibody AI325074 antibody AU045576 antibody CDC 21 antibody CDC 54 antibody CDC21 antibody CDC21 homolog antibody CDC21, S. pombe, homolog of antibody CDC54 antibody Cell division cycle 21, S. pombe, homolog of antibody DNA replication licensing factor MCM 4 antibody DNA replication licensing factor MCM4 antibody hCdc 21 antibody hCdc21 antibody Homolog of S. pombe cell devision cycle 21 antibody KIAA4003 antibody mcdc21 antibody MCM 4 antibody MCM 4 minichromosome maintenance deficient 4 antibody MCM4 antibody MCM4 minichromosome maintenance deficient 4 antibody MCM4 minichromosome maintenance deficient 4, mitotin(S. cerevisiae) antibody MCM4_HUMAN antibody Mcmd4 antibody MGC33310 antibody Minichromosome Maintenance 4 antibody Minichromosome maintenance complex component 4 antibody Minichromosome maintenance deficient(S. cerevisiae) 4 antibody Minichromosome maintenance deficient 4 antibody Minichromosome maintenance deficient 4 homolog(S. cerevisiae) antibody Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody mKIAA4003 antibody P1 CDC21 antibody P1-CDC21 antibody P1CDC21 antibody

Uniprot ID :

P33991

more info or order :

Boster Immunoleader product webpage