Boster Immunoleader

antibody

Anti-MCM3 Antibody

PA1651

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

info changed 20150724

Anti-MCM3 Antibody

PA1651

Polyclonal

Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM3(MCM3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3(13-27aa REAQRDYLDFLDDEE), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

MCM3(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (Apr 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res 20 (5): 1069–74. 3. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)".

MCM3

DNA replication licensing factor MCM3

minichromosome maintenance complex component 3

Cervical cancer proto oncogene 5 antibody DNA polymerase alpha holoenzyme associated P1 antibody DNA polymerase alpha holoenzyme associated protein P1 antibody DNA polymerase alpha holoenzyme-associated protein P1 antibody DNA replication factor MCM3 antibody DNA replication licensing factor MCM3 antibody HCC 5 antibody HCC5 antibody hRlf beta subunit antibody Human cervical cancer proto oncogene 5 antibody MCM 3 antibody mcm3 antibody MCM3 minichromosome maintenance deficient 3 antibody MCM3_HUMAN antibody MGC1157 antibody Minichromosome maintenance complex component 3 antibody Minichromosome maintenance deficient 3 antibody Minichromosome maintenance protein 3 antibody P1 h antibody P1 MCM3 antibody P1 Protein antibody P1-MCM3 antibody P1.h antibody p102 antibody P102 protein antibody Replication licensing factor beta subunit antibody RLF beta subunit antibody RLF subunit beta antibody RLFB antibody

P25205