Boster Immunoleader

antibody

Anti-Cystathionase Antibody

PA1646

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Cystathionase Antibody

PA1646

Polyclonal

Rabbit IgG polyclonal antibody for Cystathionine gamma-lyase(CTH) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Cystathionase(112-131aa DVYGGTNRYFRQVASEFGLK), different from the related rat and mouse sequences by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

CTH(CYSTATHIONINE GAMMA-LYASE),also called CYSTATHIONASE, is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate.And the CTH gene is mapped on 1p31.1. In some bacteria and mammals, including humans, this enzyme takes part in generating hydrogen sulfide and that genetic deletion of this enzyme in mice markedly reduced H2S levels in the serum, heart, aorta, and other tissues. Mutant mice lacking CTH displayed pronounced hypertension and diminished endothelium-dependent vasorelaxation. Cystathionine was physiologically activated by calcium and calmodulin, which is a mechanism for H2S formation in response to vascular activation.

1. Donald, L. J., Wang, H. S., Hamerton, J. L. Assignment of the gene for cystathionase (CYS) to human chromosome 16. 2. Ishii, I., Akahoshi, N., Yamada, H., Nakano, S., Izumi, T., Suematsu, M. Cystathionine gamma-Lyase-deficient mice require dietary cysteine to protect against acute lethal myopathy and oxidative injury. J. Biol. Chem. 285: 26358-26368, 2010. 3. Lu, Y., O'Dowd, B. F., Orrego, H., Israel, Y. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase. Biochem. Biophys. Res. Commun. 189: 749-758, 1992.

CTH

Cystathionine gamma-lyase

cystathionase(cystathionine gamma-lyase)

CGL_HUMAN antibody CTH antibody cystathionase(cystathionine gamma-lyase) antibody Cystathionine gamma lyase antibody Cystathionine gamma-lyase antibody Cysteine desulfhydrase antibody Gamma cystathionase antibody Gamma-cystathionase antibody Homoserine deaminase antibody Homoserine dehydratase antibody MGC9471 antibody

P32929