Anti-Bub3 Antibody | Boster Immunoleader PA1645 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Bub3 Antibody

catalog :

PA1645

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

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product information

Product Name :

Anti-Bub3 Antibody

Catalog Number :

PA1645

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitotic checkpoint protein BUB3(BUB3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC); though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Reference :

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C. Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. Kalitsis, P., Earle, E., Fowler, K. J., Choo, K. H. A. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 14: 2277-2282, 2000. 3. Morgan, David O (2007). The cell cycle: principles of control. London: Published by New Science Press in association with Oxford University Press.

Gene Name :

BUB3

Protein Name :

Mitotic checkpoint protein BUB3

Gene Full Name :

budding uninhibited by benzimidazoles 3 homolog(yeast)

Synonyms :

Aa2-050 antibody AU019800 antibody AU021329 antibody AU043350 antibody AW146323 antibody BUB 3 antibody BUB 3L antibody BUB3(budding uninhibited by benzimidazoles 3, yeast) homolog antibody bub3 antibody BUB3 budding uninhibited by benzimidazoles 3 homolog antibody BUB3_HUMAN antibody BUB3L antibody Budding uninhibited by benomyl antibody Budding uninhibited by benzimidazoles 3 antibody Budding uninhibited by benzimidazoles 3 homolog(S. cerevisiae) antibody Budding uninhibited by benzimidazoles 3 homolog(yeast) antibody Budding uninhibited by benzimidazoles 3 homolog antibody Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody C78067 antibody hBUB3 antibody Mitotic checkpoint component antibody Mitotic checkpoint protein bub3 antibody OTTHUMP00000020679 antibody OTTHUMP00000020680 antibody WD repeat type I transmembrane protein A72.5 antibody

Uniprot ID :

O43684

more info or order :

Boster Immunoleader product webpage