Anti-Ku80 Antibody | Boster Immunoleader PA1641 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Ku80 Antibody

catalog :

PA1641

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-Ku80 Antibody

Catalog Number :

PA1641

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for X-ray repair cross-complementing protein 5(XRCC5) detection. Tested with WB, IHC-P, ICC in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80(648-662aa KFSEEQRFNNFLKAL).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

XRCC5(X-ray Repair, Complementing Defective, In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining(NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.

Reference :

1. Blunt, T., Taccioli, G. E., Priestley, A., Hafezparast, M., McMillan, T., Liu, J., Cole, C. C., White, J., Alt, F. W., Jackson, S. P., Schurr, E., Lehmann, A. R., Jeggo, P. A. A YAC contig encompassing the XRCC5 (Ku80) DNA repair gene and complementation defective cells by YAC protoplast fusion. Genomics 30: 320-328, 1995. 2. Chen, D. J., Marrone, B. L., Nguyen, T., Stackhouse, M., Zhao, Y., Siciliano, M. J. Regional assignment of a human DNA repair gene (XRCC5) to 2q35 by x-ray hybrid mapping. Genomics 21: 423-427, 1994.

Gene Name :

XRCC5

Protein Name :

X-ray repair cross-complementing protein 5

Gene Full Name :

X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)

Synonyms :

86 kDa subunit of Ku antigen antibody ATP dependent DNA helicase 2 subunit 2 antibody ATP dependent DNA helicase II 80 kDa subunit antibody ATP dependent DNA helicase II 86 Kd subunit antibody ATP dependent DNA helicase II antibody ATP-dependent DNA helicase 2 subunit 2 antibody ATP-dependent DNA helicase II 80 kDa subunit antibody CTC box binding factor 85 kDa antibody CTC box-binding factor 85 kDa subunit antibody CTC85 antibody CTCBF antibody DNA repair protein XRCC5 antibody KARP 1 antibody KARP1 antibody Ku 80 antibody Ku autoantigen 80kDa antibody Ku80 antibody Ku86 antibody Ku86 autoantigen related protein 1 antibody KUB 2 antibody KUB2 antibody Lupus Ku autoantigen protein p86 antibody NFIV antibody Nuclear factor IV antibody Thyroid lupus autoantigen antibody Thyroid-lupus autoantigen antibody TLAA antibody X ray repair complementing defective repair in Chinese hamster cells 5(double strand break rejoining) antibody X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining) antibody X-ray repair cross-complementing protein 5 antibody Xray repair complementing defective repair in Chinese hamster cells 5 antibody XRCC 5 antibody XRCC5 antibody XRCC5_HUMAN antibody

Uniprot ID :

P13010

more info or order :

Boster Immunoleader product webpage