Boster Immunoleader

antibody

Anti-JAK2 Antibody

PA1629

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-JAK2 Antibody

PA1629

Polyclonal

Rabbit IgG polyclonal antibody for Tyrosine-protein kinase JAK2(JAK2) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human JAK2(1096-1113aa DEIYMIMTECWNNNVNQR).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Janus kinase 2, commanly called JAK2, is a human protein that has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. Exactly, JAK2 kinase is a member of a family of tyrosine kinases involved in cytokine receptor signaling. The JAK2 gene is mapped to 9p24.1. The JAK2 gene encodes a 1,132-amino acid protein that shares 95% sequence similarity to rat and pig Jak2. JAK2 is constitutively associated with the prolactin receptor and that it is activated and tyrosine phosphorylated upon PRL binding to the PRL receptor. JAK2, and more specifically just its intact N-terminal domain, binds to EPOR in the endoplasmic reticulum and promotes its cell surface expression. The human JAK2 is present in the nucleus of hematopoietic cells and directly phosphorylates tyr41 on histone H3.

1. Baxter, E. J., Scott, L. M., Campbell, P. J., East, C., Fourouclas, N., Swanton, S., Vassiliou, G. S., Bench, A. J., Boyd, E. M., Curtin, N., Scott, M. A., Erber, W. N., Cancer Genome Project, Green, A. R. Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders. Lancet 365: 1054-1061, 2005. Note: Erratum: Lancet 366: 122 only, 2005. 2. Bercovich, D., Ganmore, I., Scott, L. M., Wainreb, G., Birger, Y., Elimelech, A., Shochat, C., Cazzaniga, G., Biandi, A., Basso, G., Cario, G., Schrapper, M., and 17 others. Mutations of JAK2 in acute lymphoblastic leukaemias associated with Down's syndrome. Lancet 372: 1484-1492, 2008. 3. Bole-Feysot C, Goffin V, Edery M, Binart N, Kelly PA (1998). "Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice".

JAK2

Tyrosine-protein kinase JAK2

Janus kinase 2

JAK 2 antibody JAK-2 antibody JAK2 antibody JAK2_HUMAN antibody Janus Activating Kinase 2 antibody Janus Kinase 2 antibody JTK 10 antibody JTK10 antibody OTTHUMP00000043260 antibody THCYT3 antibody Tyrosine protein kinase JAK2 antibody Tyrosine-protein kinase JAK2 antibody

O60674