Anti-HSD17B2 Antibody | Boster Immunoleader PA1604 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HSD17B2 Antibody

catalog :

PA1604

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-HSD17B2 Antibody

Catalog Number :

PA1604

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 2(HSD17B2) detection. Tested with WB, IHC-P, IHC-F in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2(373-387aa MPRALRMPNYKKKAT).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

HSD17B2(17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42(43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Reference :

1. Berube, D., Luu-The, V., Simard, J., Gagne, R., Labrie, F. Localization of the beta-estradiol dehydrogenase genes to q11-q12 of chromosome 17. (Abstract) Cytogenet. Cell Genet. 51: 962 only, 1989. 2. Geissler, W. M., Davis, D. L., Wu, L., Bradshaw, K. D., Patel, S., Mendonca, B. B., Elliston, K. O., Wilson, J. D., Russell, D. W., Andersson, S. Male pseudohermaphroditism caused by mutations of testicular 17-beta-hydroxysteroid dehydrogenase 3. Nature Genet. 7: 34-39, 1994. 3. Harkness, R. A., Thistlethwaite, D., Darling, J. A. B., Skakkebaek, N. E., Corker, C. S. Neutral 17-beta-hydroxysteroid oxidoreductase deficiency in testes causing male pseudohermaphroditism in an infant. J. Inherit. Metab. Dis. 2: 51-54, 1979.

Gene Name :

HSD17B2

Protein Name :

Estradiol 17-beta-dehydrogenase 2

Gene Full Name :

hydroxysteroid(17-beta) dehydrogenase 2

Synonyms :

17 beta HSD 2 antibody 17-beta-HSD 2 antibody 17-beta-hydroxysteroid dehydrogenase type 2 antibody 20 alpha HSD antibody 20 alpha hydroxysteroid dehydrogenase antibody 20 alpha-hydroxysteroid dehydrogenase antibody 20-alpha-HSD antibody DHB2_HUMAN antibody E2DH antibody EDH17B2 antibody Estradiol 17-beta-dehydrogenase 2 antibody HSD17 antibody Hsd17b2 antibody Hydroxysteroid(17 beta) dehydrogenase 2 antibody Microsomal 17 beta hydroxysteroid dehydrogenase antibody Microsomal 17-beta-hydroxysteroid dehydrogenase antibody SDR9C2 antibody Testosterone 17 beta dehydrogenase antibody Testosterone 17-beta-dehydrogenase antibody

Uniprot ID :

P37059

more info or order :

Boster Immunoleader product webpage