Boster Immunoleader

antibody

Anti-Caspase 9 Antibody

PA1595

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Caspase 9 Antibody

PA1595

Polyclonal

Rabbit IgG polyclonal antibody for Caspase-9(CASP9) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Caspase 9(183-198aa SNIDCEKLRRRFSSLH).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CASP9(CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates(GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.

1. Chew, S. K., Chen, P., Link, N., Galindo, K. A., Pogue, K., Abrams, J. M. Genome-wide silencing in Drosophila captures conserved apoptotic effectors. Nature 460: 123-127, 2009. 2. Hadano, S., Nasir, J., Nichol, K., Rasper, D. M., Vaillancourt, J. P., Sherer, S. W., Beatty, B. G., Ikeda, J.-E., Nicholson, D. W., Hayden, M. R. Genomic organization of the human caspase-9 gene on chromosome 1p36.1-p36.3. Mammalian Genome 10: 757-760, 1999. 3. Kuida, K., Haydar, T. F., Kuan, C.-Y., Gu, Y., Taya, C., Karasuyama, H., Su, M. S.-S., Rakic, P., Flavell, R. A. Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9. Cell 94: 325-327, 1998.

CASP9

Caspase-9(CASP-9)

caspase 9, apoptosis-related cysteine peptidase

APAF 3 antibody APAF-3 antibody APAF3 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease activating factor 3 antibody Apoptotic protease Mch 6 antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP 9 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase 9 Dominant Negative antibody Caspase 9c antibody Caspase-9 antibody Caspase-9 subunit p10 antibody Caspase9 antibody ICE LAP6 antibody ICE like apoptotic protease 6 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody MCH6 antibody RNCASP9 antibody

P55211