Anti-Mineralocorticoid Receptor Antibody | Boster Immunoleader PA1594 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Mineralocorticoid Receptor Antibody

catalog :

PA1594

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Mineralocorticoid Receptor Antibody

Catalog Number :

PA1594

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mineralocorticoid receptor(NR3C2) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Mineralocorticoid Receptor(966-984aa DQLPKVESGNAKPLYFHRK), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

NR3C2(nuclear receptor subfamily 3, group C, member 2) also known as MR(mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. NR3C2 is a receptor with high affinity for mineralocorticoids. It belongs to the nuclear receptor family where the ligand diffuses into cells, interacts with the receptor and results in a signal transduction affecting specific gene expression in the nucleus. MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system(hippocampus), brown adipose tissue and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports(mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body. The receptor is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol. In intact animals, the mineralocorticoid receptor is "protected" from glucocorticoids by co-localization of an enzyme, 11beta-hydroxysteroid dehydrogenase type 2(11beta-HSD2), that converts cortisol to inactive cortisone. It also responds to some progestins. Spironolactone and eplerenone are mineralocorticoid receptor antagonists. Activation of the mineralocorticoid receptor, upon the binding of its ligand aldosterone, results in its translocation to the cell nucleus, homodimerization and binding to hormone response elements present in the promoter of some genes. This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes.

Reference :

1. Alnemri, E. S., Maksymowych, A. B., Robertson, N. M., Litwack, G.Overexpression and characterization of the human mineralocorticoid receptor.J. Biol. Chem. 266: 18072-18081, 1991. 2. Hellal-Levy, C., Fagart, J., Souque, A., Wurtz, J.-M., Moras, D., Rafestin-Oblin, M.-E.Crucial role of the H11-H12 loop in stabilizing the active conformation of the human mineralocorticoid receptor.Molec. Endocr. 14: 1210-1221, 2000. 3. Le Menuet, D., Isnard, R., Bichara, M., Viengchareun, S., Muffat-Joly, M., Walker, F., Zennaro, M.-C., Lombes, M.Alteration of cardiac and renal functions in transgenic mice overexpressing human mineralocorticoid receptor.J. Biol. Chem. 276: 38911-38920, 2001.

Gene Name :

NR3C2

Protein Name :

Mineralocorticoid receptor(MR)

Gene Full Name :

nuclear receptor subfamily 3, group C, member 2

Synonyms :

Aldosterone receptor antibody MCR antibody MCR_HUMAN antibody MGC133092 antibody Mineralocorticoid receptor antibody MLR antibody MR antibody NR3 C2 antibody NR3C2 antibody NR3C2 protein antibody Nuclear receptor subfamily 3 group C member 2 antibody

Uniprot ID :

P08235

more info or order :

Boster Immunoleader product webpage