Boster Immunoleader

antibody

Anti-Granzyme A Antibody

PA1588

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Granzyme A Antibody

PA1588

Polyclonal

Rabbit IgG polyclonal antibody for Granzyme A(GZMA) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Granzyme A(175-190aa DRKVCNDRNHYNFNPV).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes(CTL) and natural killer(NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are though to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A .

1. Fan, Z., Beresford, P. J., Oh, D. Y., Zhang, D., Lieberman, J.Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor.Cell 112: 659-672, 2003. Note: Erratum: Cell 115: 241 only, 2003. 2. Fink, T. M., Lichter, P., Wekerle, H., Zimmer, M., Jenne, D. E.The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily.Genomics 18: 401-403, 1993. 3. Martinvalet, D., Dykxhoorn, D. M., Ferrini, R., Lieberman, J.Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death.Cell 133: 681-692, 2008.

GZMA

Granzyme A

granzyme A(granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)

CTL tryptase antibody CTLA3 antibody Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody Cytotoxic T lymphocyte associated serine esterase 3 antibody Cytotoxic T lymphocyte proteinase 1 antibody Cytotoxic T-lymphocyte proteinase 1 antibody Fragmentin 1 antibody Fragmentin-1 antibody GRAA_HUMAN antibody Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody Granzyme A(Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) antibody Granzyme A(granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody Granzyme A antibody Granzyme A precursor antibody Granzyme-1 antibody GZMA antibody H factor antibody Hanukah factor serine protease antibody Hanukkah factor antibody HF antibody HFSP antibody TSP1 antibody

P12544